Wireless communication and embedded technology have shown rapid growth in last decade. An E-shaped slotted tri-band antenna is designed, analyzed, and proposed in this paper. The developed antenna operates for 2.4GHz, 3.5GHz and 5GHz frequencies having impedance bandwidths of 7.17%, 5.71% and 12% with an acceptable gain of 4.25dBi, 3.51dBi and 1.90dBi respectively. The efficiency for aforementioned frequencies are 74%, 75% and 80% respectively. The developed antenna utilizes FR4 as a substrate material. The presented structure is fabricated to receive the actual output parameters. There is a neighboring correlation between the software generated results and measured results. The additional vertical slots with E-shape slot offer desired return loss with optimum bandwidth for targeted resonating modes. The characteristics such as omnidirectional radiation pattern, moderate gain and adequate efficiency make the antenna an appropriate candidate for ISM band, WiMAX and WLAN applications.