To design and deploy wired and wireless secured network infrastructure, ensuring network and application security is a major challenge for any scientific organization. Such kind of organization faces security challenges to protect data, sensitive information, and assets as a lot of people are connected within their integrated network system. In this modern era, Next-Generation Hardware Firewall (NGFW) acts as an advanced security element to protect networks and applications. In this paper, our research experiments were held over the Atomic Energy Research Establishment (AERE) under Bangladesh Atomic Energy Commission (BAEC) network zone where Secured network infrastructure by configuring the NGFW firewall has been established for its network security purpose. In this paper, we have applied various network and application policy and rules over the AERE NGFW firewall. Then, we have analyzed and gathered statistical network data using the NGFW firewall in terms of different network security parameters. For this research work, we have collected data from 09 December 2020 to 09 December 2021. Further, the data are being used to evaluate the network and application security parameters of AERE. Finally, we illustrated how network and application security is being achieved by the NGFW firewall and provides future steps to embrace a better network infrastructure development for any scientific organization like AERE.