Numerous teaching and learning tools incorporate current technologies to enhance the interactivity of the learning experience, ensuring that the learners are fully engaged in the learning process. Some of the technologies adopted in the mobile-based education systems are virtual reality, augmented reality, and mixed reality. In Biology, one of the problems faced by the learners is the difficulty to visualize complex cellular processes such as cell division in the form of mitosis and meiosis. In this work, an interactive mobile learning application with virtual reality technology has been designed and developed to help learners visualize the human cell division process thus improving the understanding and interest of the learners. The mobile application which is known as InCell VR also includes mini games which can help to test the learners’ knowledge retention. The development process was done using the Agile framework based on the requirement analysis among experts and learners. The InCell VR was developed using Unity, a game engine platform for developing high performance commercial games. The final evaluation conducted among 25 learners assessed two parts: (1) the learners’ knowledge gain after using the application and; (2) the application’s usability. Overall results indicated that the proposed application had a significant positive impact on the development of learners’ knowledge. The usability study also showed that the InCell VR application is useful, easy to use, and gives satisfaction to the users.