—Over the last few years, particularly during the COVID-19 pandemic, the education ways have shifted from face-to-face learning to hybrid, blinding learning and fully online learning Human-Computer Interaction (HCI) has facilitated the development of alternative and advanced ways to implement the concept of e-learning by providing all the means that attract teachers and students to use these means by providing integrated educational content that everyone can easily access from anywhere and at any time. The research aims to measure the impact of HCI on the quality of e-learning from the point of view of the teacher and the student by measuring several factors, namely: impact of HCI on the quality of (1) teachers, (2) LMSs, and (3) learning content. To achieve the goal of the research, the researchers designed a questionnaire and used Google tools as a means of communicating with students from the target group at Tafila Technical University (TTU) to answer the questionnaire questions remotely. The data were analyzed using the SPSS program, and it was concluded that the respondents’ opinion about the effect of HCI on the quality of e-learning is positive and has an impact on all the factors described previously.